Coronavirus yog ib hom kab mob nrog lub hnab ntawv thiab cov kab ib kab ib kab zoo stranded RNA genome. Nws txoj kab uas hla yog hais txog 80-120nm. Tam sim no, nws tsuas yog kis tau cov kab mob vertebrates xws li tib neeg, nas, npua, miv, dev thiab nqaij qaib. Tus kab mob Coronavirus tshiab -19 tau raug txheeb xyuas tias yog qhov hloov pauv tshiab, uas tuaj yeem ua rau muaj kab mob ntsws, ua npaws me, hnoos qhuav, ua pa nyuaj thiab txawm tias poob siab.
Tus Tshiab Coronavirus (COVID{0}}) nucleic acid kuaj cov khoom siv (QPCR sojntsuam txoj kev) tsim los ntawm RXC tsim ob lub hom phiaj tsim raws li ORF 1ab (RDRP gene) ntawm Tus Kab Mob Coronavirus Tshiab thiab cov kev khaws cia tshwj xeeb ua ntu ntu ntawm N gene ntawm nucleocapsid protein. Siv ib-kauj ruam rov qab transcription polymerase saw cov tshuaj tiv thaiv (RT-PCR) ua ke nrog TaqMan hydrolysis sojntsuam technology, tus qauv RNA tau kuaj pom los ntawm kev hloov pauv ntawm cov teeb liab fluorescence ntawm fluorescence ntau PCR ntsuas.
PCR kuaj pom muaj xws li primers thiab sojntsuam rau cov txheej txheem genes. Cov txheej txheem ntawm cov qauv sau thiab rho tawm yog saib xyuas los ntawm kev kuaj xyuas seb tus qauv sab hauv yog qhov qub kom tsis txhob muaj cov txiaj ntsig tsis zoo.
Tus Tshiab Coronavirus (COVID{0}}) nucleic acid kuaj cov khoom siv (cov txheej txheem PCR digital) tsim los ntawm Rui Xun biology tau siv cov tshuaj tua kab mob digital PCR ua ke nrog Taqman fluorescence sojntsuam thev naus laus zis txhawm rau kuaj pom tus kabmob tshiab tshiab.
Cov qauv no tau muab tso rau ntawm kaum tawm txhiab tus roj tee uas ua los ntawm cov PCR nti. Cov tee dej tau tshwm sim nrog PCR. Tom qab cov tshuaj tiv thaiv PCR tiav lawm, cov teeb liab fluorescence ntawm txhua lub tee tau raug nyeem tawm, thiab Poisson cov ntsiab lus faib tau siv rau kev txheeb cais thiab kev tshuaj xyuas, txhawm rau ua kom tiav qhov tseeb ntawm tus kabmob tshiab (COVID{0}}) cov ntawv luam tshwj xeeb.
Ntawm lawv, covid-19 ORF 1ab kev soj ntsuam kuaj tau sau nrog FAM fluorescence, N gene detection probe tau sau nrog hex fluorescence, thiab tus qauv sab hauv tau sau nrog Cy5 fluorescence.