Chemiluminescence Immunoassay System

Vim li cas thiaj xaiv REALY?

Pab neeg tshaj lij

REALY muaj ib lub chaw tsim kho mob siab rau kev txhim kho IVD reagents. Peb cov neeg ua haujlwm technical yog lub luag haujlwm rau kev txhim kho txheej txheem, kev txhim kho txheej txheem, thiab kev tsim qauv.

Quality Assurance

Peb muaj daim ntawv pov thawj CE, ISO 13485 daim ntawv pov thawj zoo, Daim ntawv tso cai tsim khoom, thiab kev tso cai ua lag luam hauv lub tebchaws. Peb tseem tab tom npaj FDA thiab lwm daim ntawv pov thawj.

Kev Tswj Xyuas Zoo

Tswj nruj tswj xyuas kom zoo thaum lub sijhawm ua haujlwm. Peb cov khoom muaj kev nyab xeeb raug kuaj xyuas los ntawm kev sim ntau, kuaj thiab kuaj xyuas cov txheej txheem kom ua tau raws li cov khoom lag luam kawg.

Muag rau ntiaj teb

Peb koom nrog kev nthuav qhia kho mob hauv ntau lub tebchaws xws li Medlab, Medica, thiab AACC txhua xyoo. Kev muag khoom network tau npog ntau dua 80 lub teb chaws thiab cheeb tsam, faib cov khoom zoo ntawm cov nqi sib tw heev.

Tsev 12 Rau sab kawg 1/2

Chemiluminescence Immunoassay (CLIA) yog dab tsi?

CLIA yog ib qho txheej txheem los txiav txim siab cov qauv ntsuas raws li qhov siv ntawm lub teeb tawm los ntawm cov tshuaj lom neeg thiab tshuaj lom neeg. Cov kab ke chemiluminescence (CL) thiab kev tiv thaiv kab mob sib xyaw ua ke hauv CLIA. Qee cov tshuaj tau siv los ua CL daim ntawv lo, thiab lub kaw lus tsim chemiluminescence thaum ntxiv CL substrates, tso cai rau cov qauv ntsuas. Feem ntau CL substrates muaj xws li luminol, lawv cov derivatives, alkaline phosphatase (ALP), peroxidase, thiab acridinium ester compounds. Hauv CLIA, cov enzyme kuj tseem siv rau qhov cim ntawm lub hom phiaj proteins. ALP thiab horseradish peroxidase yog dav siv rau enzyme labeling.

SARS-Cov-2 & FLU A&B & RSV & Adv Combo Rapid Test Cassette (swab)

Txoj Cai ntawm CLIA

Chemiluminescence immunoassay muaj ob lub tshuab, immunoassay thiab chemiluminescence assay. Lub tshuab immunoassay siv cov tshuaj chemiluminescent lossis enzymes ua cov cim, uas tau sau ncaj qha rau ntawm cov tshuaj tiv thaiv lossis tshuaj tiv thaiv kab mob, thiab cov tshuaj tiv thaiv kab mob antigen-antibody yog tsim los ntawm cov tshuaj tiv thaiv ntawm antigen thiab cov tshuaj tiv thaiv. Lub chemiluminescence tsom xam yog ib tug luminescent substrate ntxiv nrog ib tug oxidant los yog ib tug enzyme tom qab cov tshuaj tiv thaiv kab mob. Tom qab cov tshuaj chemiluminescent yog oxidized los ntawm oxidant, ib qho nruab nrab hauv lub xeev zoo siab yog tsim, uas emits photons tso tawm lub zog rov qab mus rau hauv av ruaj khov. Lub luminescence siv tau tuaj yeem kuaj tau siv lub teeb liab luminescence ntsuas ntsuas. Raws li kev sib raug zoo ntawm chemiluminescence marker thiab luminescence siv, cov ntsiab lus ntawm cov tshuaj ntsuam xyuas tuaj yeem suav nrog siv tus qauv nkhaus.

Peb Qhov Sib Txawv Label Systems ntawm CLIA

CLIA muaj peb hom ntawv sib txawv raws li qhov sib txawv ntawm lub cev chemistry mechanism ntawm lub teeb emission:

Label Chemical ncaj qha koom nrog hauv lub teeb Emission Reaction
Cov tshuaj no nrog cov qauv tshwj xeeb tuaj yeem hloov mus rau lub xeev zoo siab los ntawm cov tshuaj tiv thaiv tshuaj. Photons yuav raug tso tawm thaum cov tshuaj poob rau hauv av los ntawm lub xeev zoo siab. Cov tshuaj nquag yog acridinium ester thiab nws cov derivatives. Kev nthuav tawm ntawm acridinium ester daim ntawv lo rau cov tshuaj alkaline hydrogen peroxide ua rau lub teeb ci. Ib qho kev txhim kho tom ntej yog acridinium sulfonamide ester cov ntawv. Nws kuj tseem tshwm sim los ntawm alkaline hydrogen peroxide kom tawm lub teeb ci.

Enzyme Catalyzed Lub Teeb Emission Reaction
Hom chemiluminescence no siv cov enzymes los sau cov tshuaj tiv thaiv kab mob. Kev hais lus, nws yog ib qho enzyme txuas immunoassay uas siv cov tshuaj luminescent li substrate es tsis txhob chromogen. Cov enzymes uas siv ntau tshaj plaws yog horseradish peroxidase (HRP) thiab alkaline phosphatase (AP), txhua tus muaj nws cov luminescent substrates. Luminol yog ib qho chemiluminescent substrate ntau heev siv los kuaj xyuas HRP. HRP catalyzes lub decomposition ntawm luminol nyob rau hauv lub xub ntiag ntawm peroxide los tsim ib tug zoo siab lub xeev intermediate. Flashes ntawm pom lub teeb (qhov siab tshaj plaws ntawm 425nm) yog tawm ntawm kev lwj ntawm lub singlet nruab nrab.

Redox Reaction Mediated Light Emission Reaction
Lwm qhov CL system yog qhov tsim nyog vim tias cov reagent tau rov tsim dua thiab yog li tuaj yeem rov ua dua. Cov txheej txheem no siv ruthenium tris-bipyridine (bpy) raws li daim ntawv lo, suav nrog cov tshuaj tiv thaiv ntawm Ru(bpy)33+ thiab Ru(bpy){2}} los tsim lub xeev zoo siab ntawm Ru(bpy)32+ , ib hom tsiaj ruaj khov uas faus rau hauv av hauv lub xeev los ntawm emission 620nm txiv kab ntxwv emission. Ru(bpy)33+ thiab Ru(bpy)3+ tuaj yeem tsim hluav taws xob los ntawm Ru(bpy)32+ los ntawm kev txo qis ntawm kwv yees li -1.3 V, thiab oxidation ntawm kwv yees li { {10}}.3 V. Qhov system no tau mob siab rau electrochemiluminescence nrog ultrahigh rhiab heev thiab tshwj xeeb.

Qhov zoo ntawm Chemiluminescence Immunoassay System

Siab rhiab heev
rhiab heev yog tus yuam sij rau kev ua tau zoo tshaj ntawm immunoassay analyzer. Qhov rhiab heev ntawm qee cov tshuaj ntsuam xyuas tuaj yeem ncav cuag 10 -16 mol / L (RIA rau 10-12 mol / L). Lwm qhov piv txwv yog chemiluminescent substrates (xws li AMPPD), cov concentration ntawm alkaline phosphatase tuaj yeem kuaj pom dua li cov chromogenic substrate kom rhiab heev 5 х 10 ^ 5 zaug.

Wide Linear Kinetic Range
Lub luminescence siv ntawm chemiluminescence immunoassay yog linear ntawm 4 thiab 6 qhov kev txiav txim ntawm qhov loj nrog rau qhov concentration ntawm cov khoom ntsuas. Qhov no yog qhov zoo dua piv rau qhov nqus (OD) ntau ntawm 2.0 rau colorimetric enzyme immunoassays.

Human Monkeypox Virus (MPV) Real Time PCR Assay Kit

Lub Sij Hawm Ntev ntawm Lub Teeb Pom Kev

Lub ci ci hom CLIA ua rau lub teeb pom kev zoo uas kav ntev li ib teev lossis ib hnub. Qhov no yeej yooj yim rau kev sim ua haujlwm thiab ntsuas.

Txoj kev tsom xam yog yooj yim thiab ceev

Feem ntau ntawm cov chemiluminescence immunoassay analyzers yog ib-kauj ruam hom uas tsuas yog yuav tsum tau ntxiv ntawm ib tug reagent (los yog ib tug ua ke ntawm reagents).

Cov txiaj ntsig ruaj khov, qhov yuam kev me me

Cov qauv ncaj qha emits lub teeb ntawm nws tus kheej, tsis muaj teeb pom kev zoo irradiation, tshem tawm qhov cuam tshuam ntawm ntau yam ua tau (lub teeb ci ruaj khov, lub teeb tawg, lub teeb yoj xaiv, thiab lwm yam) rau kev tsom xam. Qhov no ua rau cov kev soj ntsuam tau rhiab heev, ruaj khov thiab txhim khu kev qha.

Kev nyab xeeb zoo thiab kev pab lub neej ntev

Chemiluminescent immunoassay analyzer tshem tawm kev siv cov tshuaj radioactive. Txog tam sim no, tseem tsis tau pom tias muaj kev phom sij. Ntxiv mus, cov reagents siv rau immunoassay yog ruaj khov thiab tuaj yeem khaws cia ntev txog ib xyoos.

Cheebtsam ntawm CLIA

96-zoo microtiter phaj thiab sealers
Qauv Diluent
Kev ntes thiab/lossis kuaj cov tshuaj tiv thaiv
Avidin / HRP Conjugate
Ntxuav Tsis
Substrate daws
Cov txheej txheem nthuav dav

Kev siv Chemiluminescence Immunoassay System

01/

Clinical Diagnostics:CLIA tau siv dav hauv cov chaw kuaj mob los kuaj xyuas ntau yam kab mob thiab kev kho mob. Nws tuaj yeem ntsuas biomarkers, cov tshuaj hormones, tshuaj tiv thaiv kab mob, thiab lwm yam tshuaj ntsuam xyuas qhia txog cov kab mob tshwj xeeb.

02/

Endocrinology:Nws yog feem ntau siv los ntsuas cov thyroid ua haujlwm, qib ntawm cov tshuaj hormones, thiab qib qog adrenal, pab rau kev kuaj mob thiab tswj cov kab mob endocrine.

03/

Therapeutic Drug Monitoring (TDM):CLIA yog ua hauj lwm hauv TDM los ntsuas cov tshuaj concentration hauv cov ntshav, kom ntseeg tau tias cov tshuaj nyob hauv qhov kev kho kom zoo.

04/

Allergy thiab Immunology:CLIA yog siv los txheeb xyuas cov tshuaj tiv thaiv kab mob tshwj xeeb IgE hauv cov ntshav, pab txhawm rau txheeb xyuas qhov ua xua. Nws kuj tseem siv los ntsuas lub cev tiv thaiv kab mob thiab qib tshuaj tiv thaiv kab mob hauv ntau yam kab mob tiv thaiv kab mob.

05/

Kev kuaj kab mob:CLIA yog dav siv los kuaj xyuas cov kab mob sib kis, xws li kab mob, kab mob, thiab kab mob cab, hauv kev kuaj mob.

06/

Kev kuaj kab mob autoimmune:CLIA yog siv los kuaj autoantibodies cuam tshuam nrog cov kab mob autoimmune, xws li mob caj dab rheumatoid, kab mob lupus erythematosus, thiab ntau yam sclerosis.

07/

Oncology thiab Cancer Biomarkers:CLIA yog siv rau hauv oncology los kuaj cov qog nqaij hlav thiab cov qog nqaij hlav tshwj xeeb antigens.

08/

Reproductive Health:CLIA yog siv nyob rau hauv kev noj qab haus huv ntawm cov me nyuam los ntsuas cov tshuaj hormones hais txog fertility thiab cev xeeb tub.

09/

Kev Ntsuas Tshuaj:CLIA tau ua haujlwm hauv cov kev kuaj tshuaj, tshuaj ntsuam xyuas tshuaj hauv chaw ua haujlwm, thiab kev soj ntsuam tshuaj toxicology los kuaj xyuas cov tshuaj tsim txom lossis tshuaj kho hauv cov qauv tshuaj lom neeg, xws li zis thiab ntshav.

10/

Kev Tshawb Fawb Txog Kev Kho Mob:CLIA yog ib qho cuab yeej tseem ceeb hauv kev tshawb fawb txog kev kho mob kom muaj nuj nqis biomolecules thiab kawm txog kab mob mechanisms. Nws pab kom nkag siab txog kab mob kev loj hlob, txheeb xyuas cov biomarkers tshiab, thiab ntsuas cov kev pab cuam kho mob.

Cov txheej txheem ntawm Chemiluminescence Immunoassay System

Txhais qhov Expected Dynamic Range

Nws yog ib qho tseem ceeb kom paub qhov kwv yees qhov concentration ntau ntawm cov tshuaj ntsuam xyuas hauv koj tus qauv. Tsis tas li ntawd, koj yuav tsum paub seb qhov zoo tshaj plaws concentration ntau npaum li cas tau pom zoo txhawm rau txhawm rau kuaj xyuas koj qhov kev xav tau.

Txhais cov Assayed Specimen

Hom qauv (xws li ntshav, ntshav, zis, thiab lwm yam) yuav pab koj nkag siab txog qhov kev xav tau ntawm cov tshuaj ntsuam xyuas uas muaj nyob rau hauv cov qauv.

Xaiv cov Antibodies uas yuav tsum tau siv nyob rau hauv Assay

Koj yuav xav xaiv cov tshuaj tiv thaiv kab mob uas tshwj xeeb rau koj cov tshuaj ntsuam xyuas tshwj xeeb. Qhov tshwj xeeb yog qhov tseem ceeb rau kev ua tiav ntawm koj qhov kev xeem.

Xaiv Koj Qhov Zoo Tshaj Plaws Hlau nplaum

Koj yuav tsum tau txiav txim siab seb qhov loj npaum li cas ntawm cov hlaws loj, cov hlaws saum npoo thiab cov ntsiab lus hlau oxide yuav tsum yog rau koj qhov kev ntsuam xyuas tshwj xeeb.

Optimize txheej txheej txheej txheej

Thaum txheej koj cov hlaws dai, koj yuav xav coj mus rau hauv tus account lub adsorption cov khoom ntawm cov hlaws dai, ib qho covalent khi koj yuav tsum tau tsim thiab cov teebmeem lom ntawm kev sib txuas cov proteins rau koj cov hlaws dai, lossis yog tias koj xav tau bio-activated sawv daws yuav.

Xaiv Koj Txoj Kev Homogenization

Koj qhov kev xaiv ntawm homogenization yuav nyob ntawm seb cov khoom koj muaj, cov neeg ua haujlwm ua haujlwm ntawm cov khoom siv, qhov loj ntawm koj cov qauv thiab qhov yooj yim ntawm kev siv hauv qhov kev ntsuam xyuas.

Xaiv qhov raug Biomagnetic Separation Technology

Lub tshuab no pab tshem tawm cov tshuaj tiv thaiv dawb thiab lwm yam kab mob. Cov txheej txheem uas tuaj yeem pab koj yooj yim txheeb xyuas koj cov txheej txheem thiab tuaj yeem pab koj ntsuas koj cov txheej txheem yog nyiam.

Txhim kho koj cov tshuaj tiv thaiv kab mob thaum xav tau

Yog tias koj tuaj yeem ua kom koj qhov kev ntsuam xyuas zoo dua, nws tseem ceeb heev uas yuav tsum ua li ntawd. Ua kom koj qhov kev ntsuam xyuas tau zoo dua, muaj tseeb dua thiab ua tau yooj yim dua yog qhov tseem ceeb rau qhov kawg ntawm koj qhov kev tshuaj ntsuam.

Kev txhais cov ntsiab lus

1.Lub neej ntawd concentration unit ntawm qhov kev ntsuam xyuas no yog ng/mL los yog nmol/L.
Kev hloov pauv yam:

o ng/mL x 2 5=nmol/L
o nmol/L x 0 4= ng/mL

2.Vim cov txheej txheem sib txawv los yog cov tshuaj tiv thaiv tshwj xeeb, tej zaum yuav muaj qhov sib txawv ntawm cov txiaj ntsig ntawm cov tshuaj reagents los ntawm cov tuam txhab sib txawv. Yog li ntawd, kev sib piv ncaj qha yuav tsum tsis txhob ua kom tsis txhob muaj kev txhais lus cuav.

3.Thaum lub 25-OH VD concentration nyob rau hauv cov qauv ntau tshaj 100.00 ng/mL, ib tug qauv dilution yuav tsum tau ua ua ntej ntsuas (pom zoo 2- zaug dilution).

4.Txhua yam tshwm sim hauv qab qhov kev txwv tsawg kawg nkaus yuav raug tshaj tawm raws li<3.00 ng/mL; any result above the maximum detection limit will be reported as >1 00.00 ng/mL.

Kev Tiv Thaiv Kev Tiv Thaiv

Tsis txhob siv cov khoom siv reagent dhau hnub tas sij hawm.

Tsis txhob hloov cov khoom siv reagent los ntawm cov khoom sib txawv lossis ntau ntau.

Ua ntej yuav thauj cov khoom siv Reagent rau ntawm lub kaw lus thawj zaug, Cov Khoom Siv Reagent yuav tsum tau sib tov kom rov ncua cov khoom sib nqus microbeads uas tau txiav txim siab thaum xa khoom. Rau cov lus qhia sib nqus microbeads, xa mus rau Kev Npaj ntawm Reagent seem ntawm cov pob no ntxig.

Txhawm rau kom tsis txhob muaj kab mob, hnav cov hnab looj tes huv thaum ua haujlwm nrog cov khoom siv reagent thiab cov qauv.

Nyob rau tib lub sijhawm, cov kua uas seem yuav qhuav rau ntawm qhov chaw septum. Cov no feem ntau yog cov ntsev qhuav uas tsis muaj kev cuam tshuam rau kev soj ntsuam kev ua tau zoo.

Txhawm rau kom tsis txhob evaporation ntawm cov kua hauv cov khoom siv reagent qhib hauv lub tub yees, nws raug nquahu tias cov khoom siv qhib reagent yuav tsum tau muab kaw nrog cov reagent seals uas muaj nyob rau hauv lub ntim. Cov reagent seals yog "siv ib zaug," thiab yog tias xav tau ntau cov ntsaws ruaj ruaj, thov hu rau peb.

Peb daim ntawv pov thawj

Peb tau dhau ntau daim ntawv pov thawj xws li CE thiab ISO, thiab cov khoom peb muab muaj kev ruaj ntseg zoo.

Tej teeb meem ntawm Chemiluminescence Immunoassay System

Q: Cov txheej txheem chemiluminescence immunoassay yog dab tsi?

A: Chemiluminescence immunoassay (CLIA) yog ib qho kev ntsuam xyuas uas muab cov txheej txheem chemiluminescence nrog cov tshuaj tiv thaiv kab mob. Zoo ib yam li lwm cov tshuaj tiv thaiv kab mob (RIA, FIA, ELISA), CLIA siv cov tshuaj tiv thaiv kab mob uas tuaj yeem tsim lub teeb emissions los ntawm cov tshuaj tiv thaiv rau cov tshuaj tiv thaiv kab mob.

Q: Dab tsi yog chemiluminescence immunoassay analyzer siv rau?

A: Chemiluminescent immunoassay analyzer 4 qhov kev kuaj mob ntau
Kev kuaj mob qog nqaij hlav.
Thyroid function test.
Kev kuaj mob endocrine hormone.
Kev kuaj kab mob sib kis.

Q: Qhov txawv ntawm ELISA thiab CLIA yog dab tsi?

A: Hauv kev sib piv ntawm ELISA thiab CLIA, CLIA yog qhov rhiab heev. ELISA ntsuas qhov muag pom qhov muag, qhov CLIA ntsuas qhov sib txheeb lub teeb. Raws li kev tshawb nrhiav ntau yam, CLIA ntau yam yog siab piv rau ELISA. CLIA yog qhov kev sim sai, thaum ELISA siv sijhawm. ELISA yog ib qho kev ntsuas tus nqi zoo li piv rau CLIA. Dhau li ntawm cov kev sib piv no, cov kev tshawb fawb yav dhau los kuj tau tshaj tawm tias CLIA zoo dua piv rau ELISA.

Q: chemiluminescence assay ua haujlwm li cas?

A: Cov enzymes siv nyob rau hauv chemiluminescent microparticle immunoassay hloov lub substrate rau cov tshuaj tiv thaiv cov khoom, uas emits ib photon ntawm lub teeb es tsis txhob tsim ib tug tshwj xeeb xim. Luminescence txhais tau hais tias lub teeb tawm los ntawm ib yam khoom thaum nws rov qab los ntawm lub xeev zoo siab mus rau hauv av.

Q: Dab tsi yog hom chemiluminescence immunoassay?

A: daim ntawv lo antigens lossis cov tshuaj tiv thaiv nrog cov tshuaj chemiluminescence. Chemiluminescence enzyme immunoassay yog ib qho enzyme-labeled immunoassay uas siv cov tshuaj chemiluminescence ua substrates rau cov tshuaj tiv thaiv enzyme. Microparticle chemiluminescence immunoassay muaj ob txoj hauv kev. Electrochemiluminescence immunoassay yog ib qho tshuaj tiv thaiv luminescence pib los ntawm electrochemistry ntawm electrode nto.

Q: CLIA ua haujlwm li cas?

A: Chemiluminescent immunoassays (CLIA) muab cov txiaj ntsig zoo ntawm chemiluminescence rhiab heev nrog rau qhov tshwj xeeb ntawm kev tiv thaiv kab mob. Nyob rau hauv cov txheej txheem no, ib tug enzyme hloov lub substrate rau hauv ib tug chemiluminescent teeb liab uas yog emitted raws li photons ntawm lub teeb nyob rau hauv lub pom los yog ze pom ntau yam.

Q: Yuav ua li cas yog chemiluminescence immunoassay?

A: Ntawm qhov chaw tsim khoom tus nqi txiav tawm ntawm 10 AU / mL, rhiab heev yog 73.3% thiab 76.7% thiab qhov tshwj xeeb yog 92.2% thiab 100% rau IgM thiab IgG cov tshuaj tiv thaiv, feem. Peb tau tshaj tawm plaub qhov txiaj ntsig zoo ntawm IgM hauv pawg tswj hwm: ob kis ntawm tus kab mob cytomegalovirus, ib tus kab mob scleroderma, thiab ib tus neeg mob lupus erythematosus.

Q: Lub hom phiaj ntawm chemiluminescence yog dab tsi?

A: Cov txheej txheem ntawm chemiluminescence yog dav siv hauv kev lag luam kws tshuaj los tshuaj xyuas kev sib kis ntawm cov tshuaj lom neeg thiab tshuaj xyuas cov impurities hauv cov tshuaj. Tsis tas li ntawd, cov txheej txheem kuj tseem siv los ntsuas qib ntawm cov tshuaj hormones. Thaum kawg chemiluminescence kuj tseem siv los kuaj xyuas ntau yam tshuaj hauv lub cev.

Q: chemiluminescence tsom xam yog dab tsi?

A: Luminescence yog qhov tshwm sim uas qhov teeb meem emits lub teeb ntawm ib lub wavelength tshwj xeeb tsis muaj cua sov thiab rov qab mus rau hauv av los ntawm lub xeev zoo siab tom qab tau nqus cov hluav taws xob sab nraud los ntawm hluav taws xob, hluav taws xob, hluav taws xob, kev sib txhuam, hluav taws xob, lossis tshuaj lom neeg cov tshuaj tiv thaiv.

Q: Vim li cas CLIA zoo dua ELISA?

A: Txawm hais tias ELISA tsis tuaj yeem nyeem tau siab dua 3.0 OD yog li xav tau cov qauv dilution, CLIA tuaj yeem nyeem qhov ntau ntawm 106 lossis 107 ua rau muaj kev ntsuam xyuas ntau dua thiab txo qis. Tsis tas li ntawd, tsis tshua muaj keeb kwm yav dhau (siab teeb liab-rau-suab nrov piv) ua kom pom tseeb dua ntawm qhov ntsuas qis qis.

Q: Puas yog CLIA yog ELISA?

A: Enzyme Linked Immunosorbent Assay (ELISA) thiab Chemiluminescence Immunoassay (CLIA) yog ob qhov kev sim nquag ua haujlwm rau kom muaj nuj nqis ntawm Anti-HBs, ntawm lwm yam kev sim, suav nrog Radioimmunoassay thiab micro-particle enzyme immunoassay (MEIA). Txawm li cas los xij, ELISA thiab CLIA yog raws li cov qauv xeem sib txawv.

Q: Puas yog CLIA yog ib qho kev kuaj mob immunoassay?

A: Lub hauv paus ntawm CLIA txoj kev zoo ib yam li ELISA, tshwj tsis yog tias CLIA substrates tuaj yeem tsim lub teeb emission nyob rau hauv muaj cov enzyme, muab cov txheej txheem rhiab dua piv rau ELISA. Substrates xws li isoluminol lossis acridinium ester tsim cov teeb liab luminescence nyob rau hauv muaj hydrogen peroxide thiab enzyme. Lub electro-chemiluminescence immunoassay (ECLIA), lwm hom CLIA, siv hluav taws xob tam sim no rau oxidizing substrate.

Q: Dab tsi yog qhov zoo ntawm chemiluminescence assay?

A: Qhov zoo ntawm kev siv chemiluminescence hauv kev tiv thaiv kab mob muaj xws li kev xav tau siab, ruaj khov, thiab tus neeg teb xov tooj yooj yim. Qhov tsis zoo tsis tau hais hauv cov ntawv nyeem. Qhov zoo: siab rhiab heev, dav linear dynamic ntau, ntev-ntev optical teeb liab.

Q: Dab tsi yog chemiluminescence immunoassay vs fluorescence?

A: Qhov sib txawv tseem ceeb ntawm chemiluminescence thiab fluorescence yog tias chemiluminescence yog lub teeb tawm los ntawm cov tshuaj tiv thaiv tshuaj, qhov fluorescence yog lub teeb tawm los ntawm kev nqus ntawm lub teeb lossis hluav taws xob hluav taws xob.

Q: Cov khoom ntawm chemiluminescence immunoassay yog dab tsi?

A: Chemiluminescence immunoassay yog ib qho cuab yeej immunoassay uas ncaj qha sau cov tshuaj tiv thaiv kab mob lossis tshuaj tiv thaiv kab mob nrog tus neeg sawv cev chemiluminescent. Chemiluminescent immunoassay analyzer muaj ob ntu, piv txwv li lub cev tiv thaiv kab mob thiab chemiluminescent tsom xam.

Q: Puas yog immunoassay thiab ELISA tib yam?

A: ELISA (enzyme-linked immunosorbent assay) yog cov txheej txheem tshuaj ntsuam xyuas phaj tsim los kuaj xyuas thiab ntsuas cov tshuaj soluble xws li peptides, proteins, tshuaj tiv thaiv kab mob, thiab cov tshuaj hormones. Lwm cov npe, xws li enzyme immunoassay (EIA), kuj tau siv los piav txog tib lub tshuab.

Q: Vim li cas peb thiaj xav tau CLIA?

A: Lub Tsev Kho Mob Kho Kho Kho Kho Kho Kho Kho Kho Kho Kho Mob (CLIA) tswj cov chaw soj ntsuam kuaj tib neeg cov qauv thiab xyuas kom lawv muab cov txiaj ntsig zoo, txhim khu kev qha, thiab raws sij hawm tus neeg mob cov txiaj ntsig tsis hais qhov twg qhov kev sim ua tiav. CMS saib xyuas txhua qhov kev kuaj sim (tshwj tsis yog qee qhov kev tshawb fawb) ua rau tib neeg hauv Asmeskas los ntawm CLIA.

Q: Puas yog CLIA kuaj pom zoo?

A: CLIA, txoj kev tsim kho tshiab, tuaj yeem tso cai rau kev tshawb pom sai ntawm T. pallidum cov tshuaj tiv thaiv tshwj xeeb ntawm cov neeg soj ntsuam random-access nrog ntau dua precision, rhiab heev thiab tshwj xeeb. Thiab TPPA, raws li kev kuaj pom tseeb, tuaj yeem ua kom ntseeg tau tias nws qhov kev nkag siab zoo thiab tshwj xeeb rau kev kuaj mob syphilis los ntawm kev siv T.

Q: Dab tsi yog qee qhov tsis zoo ntawm chemiluminescence?

A: Cons ntawm chemiluminescence assay muaj xws li:
Limited Ag nrhiav kom tau.
Kaw analytical systems.
Txwv kev xeem vaj huam sib luag.
Cov nqi siab dua.

Q: Dab tsi yog txhim kho chemiluminescence immunoassay?

A: Enhanced chemiluminescence (ECL) yog cov txheej txheem tshawb nrhiav raws li chemiluminescence ntawm substrates xws li luminol thiab acridan. Vim nws lub siab rhiab heev, dav dynamic ntau, thiab siab teeb liab-rau-suab piv, ECL yog ib qho ntawm feem nrov nrhiav kom tau txoj kev rau ntau yam western blotting daim ntaub ntawv, thiab kuj yog lug siv rau quantifying lom analytes xws li DNA, RNA. zoo li cov hlwb.

Q: Qhov txawv ntawm CLIA thiab Cleia yog dab tsi?

A: CLIA siv CL los sau cov tshuaj tiv thaiv thiab los ntawm kev qhia CL substrates, lub kaw lus tsim CL; yog li ntawd, cov qauv tuaj yeem soj ntsuam kom muaj nuj nqis. Acridinium ester derivative tau siv los ua lub substrate rau CL, thaum CLEIA siv peroxidase los sau cov tshuaj tiv thaiv kab mob thiab luminol ua substrate.

Raws li ib qho ntawm cov kws tshaj lij chemiluminescence immunoassay system tuam ntxhab thiab cov muag khoom hauv Suav teb, peb tau tshwj xeeb los ntawm cov khoom zoo thiab cov kev pabcuam zoo. Thov so assured mus ua lag luam wholesale pheej yig chemiluminescence immunoassay system los ntawm peb lub hoobkas. Tiv tauj peb kom paub meej ntxiv.