Chemiluminescence Immunoassay, CLIA yog cov txheej txheem immunoassay rhiab heev.
1. Txoj Cai
Chemiluminescence immunoassay ua ke qhov tshwj xeeb ntawm lub cev tiv thaiv kab mob thiab qhov siab rhiab heev ntawm chemiluminescence. Nws lub hauv paus ntsiab lus yog los ua ke cov kev tiv thaiv kab mob tshwj xeeb ntawm antigen thiab cov tshuaj tiv thaiv kab mob nrog cov tshuaj tiv thaiv chemiluminescence. Tshwj xeeb, cov antigen hauv cov qauv yuav tsum tau kuaj ua ke nrog cov tshuaj tiv thaiv tshwj xeeb los tsim cov tshuaj tiv thaiv kab mob. Tom qab ntawd, dhau ntawm cov tshuaj tiv thaiv kab mob, lub cev tiv thaiv kab mob ua rau lub teeb liab chemiluminescent. Lub luminescence siv yog proportional rau qhov concentration ntawm cov antigen yuav tsum tau kuaj, thiab cov ntsiab lus ntawm cov antigen yuav tsum tau kuaj yuav quantitatively soj ntsuam los ntawm kev soj ntsuam cov luminescence siv.
2. Cov khoom tseem ceeb
Antibodies: suav nrog kev ntes cov tshuaj tiv thaiv thiab kuaj cov tshuaj tiv thaiv. Kev ntes cov tshuaj tiv thaiv kab mob feem ntau yog kho rau ntawm cov khoom thauj khoom thauj mus rau kev ntes cov antigen los kuaj. Kev kuaj pom cov tshuaj tiv thaiv yog ua ke nrog chemiluminescent marker los kuaj xyuas cov antigen hauv lub cev tiv thaiv kab mob.
Cov khoom thauj khoom theem: xws li microplates, cov hlaws sib nqus, thiab lwm yam, siv los kho cov tshuaj tua kab mob los pab txhawb kev tiv thaiv kab mob thiab kev sib cais thiab ntxuav.
Chemiluminescent marker: feem ntau yog ib yam khoom uas tuaj yeem tsim cov teeb liab luminescent nyob rau hauv cov tshuaj tiv thaiv, xws li luminol, acridinium ester, thiab lwm yam. Cov cim qhia ua ke nrog cov tshuaj tiv thaiv kev tshawb nrhiav los tsim cov teeb liab chemiluminescent tom qab kev tiv thaiv kab mob.
Kev kuaj xyuas qhov system: suav nrog cov khoom siv xws li luminescence detectors, uas yog siv los xyuas qhov kev siv ntawm cov teeb liab chemiluminescent thiab hloov lawv mus rau qhov tseem ceeb nyeem tau.
3. Nta
Siab rhiab heev: Nws tuaj yeem kuaj pom qhov tsis tshua muaj siab ntawm cov tshuaj ntsuam xyuas, uas feem ntau yog ntau qhov kev txiav txim siab ntau dua li cov txheej txheem immunoassay.
Wide linear range: Cov ntsiab lus ntawm cov tshuaj ntsuam xyuas tuaj yeem ntsuas qhov tseeb hauv qhov dav dav yam tsis muaj ntau yam dilutions.
Muaj zog tshwj xeeb: Raws li kev khi tshwj xeeb ntawm cov tshuaj tiv thaiv kab mob thiab cov tshuaj tiv thaiv kab mob, nws muaj kev xaiv zoo rau cov tshuaj ntsuam xyuas tshwj xeeb.
Fast: Cov txheej txheem tshawb nrhiav tau nrawm thiab cov txiaj ntsig feem ntau tuaj yeem tau txais hauv lub sijhawm luv dua.
Qib siab ntawm automation: Nws yog qhov tsim nyog rau kev kuaj xyuas cov qauv loj thiab tuaj yeem siv nrog cov cuab yeej siv tshuab los txhim kho kev tshawb pom.
4. Cov chaw thov
Kev kuaj mob hauv tsev kho mob: Nws yog siv los txheeb xyuas ntau yam biomarkers, xws li cov cim qog, cov tshuaj hormones, cov cim kab mob sib kis, thiab lwm yam, los muab lub hauv paus tseem ceeb rau kev kuaj kab mob, kev saib xyuas kev kho mob thiab kev ntsuas kev ntsuas.
Kev txhim kho tshuaj: Nws yog siv rau kev tshuaj xyuas cov tshuaj concentration, tshuaj xyuas kev ua tau zoo thiab kev tshawb fawb tshuaj metabolism.
Kev soj ntsuam kev nyab xeeb ntawm cov khoom noj: Kev kuaj xyuas cov khoom tsis zoo, cov tshuaj tua kab residues, cov tshuaj veterinary residues, thiab lwm yam hauv cov zaub mov.
Kev saib xyuas ib puag ncig: Tshawb xyuas cov pa phem thiab cov tshuaj lom hauv ib puag ncig.