Electrochemical biosensor rau kev kuaj mob covid-19 raws li cov protein ntau
Sars-cov-2 yog tsim los ntawm plaub yam qauv proteins: spike protein, nucleocapsid protein, hnab ntawv protein thiab membrane protein (raws li qhia hauv daim duab 1). Ntawm lawv, spike protein yog lub hom phiaj protein uas tuaj yeem siv los kuaj xyuas covid-19. Nws muaj kev tiv thaiv kab mob siab thiab receptor binding domain (RBD). Yog li ntawd, nws yog ib qho tseem ceeb heev uas yuav tau siv cov protein ntau ntawm sars-cov-2 hauv kev kuaj mob covid-19 thiab kev tsim cov tshuaj tiv thaiv thiab cov tshuaj kho mob. Raws li qhov no, ntau tus kws tshawb fawb tau tsim ntau yam electrochemical biosensors los kuaj sars-cov-2.
Lub graphene electrode ntawm FET tau hloov kho nrog 1-Pyrene butyric acid N-hydroxysuccinimide ester (pbase), uas tau siv los ua tus neeg sawv cev sib txuas los kho cov sars-cov-2 spike protein tshwj xeeb antibody (raws li qhia hauv daim duab 2a), thiab tom qab ntawd FET tau them tag nrho nrog phosphate buffered saline (PBS, pH 7.4) raws li cov electrolyte. Cov qauv kuaj mob kuaj pom ntawm sars-cov-2 tau paub raws li qhov muaj peev xwm hloov pauv ntawm cov kav dej thiab qhov cuam tshuam ntawm cov lus teb hluav taws xob, Qhov kev kuaj pom qhov txwv tsis pub kuaj pom yog 2.42 × 102 daim ntawv luam / mL. Electrochemical biosensor raws li FET muaj qhov zoo ntawm tus nqi qis, siab rhiab heev thiab dav dynamic teb ntau yam.